Primer

Part:BBa_K5427051

Designed by: Brittany Green   Group: iGEM24_UAlberta   (2024-09-23)


FW_pJump_24_RBS4_sfGFP (I GEM_2024_30)

Forward primer for Gibson Assembly of pTac RBS3 sfGFP in pJUMP24.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 11
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 12
    Illegal PstI site found at 26
    Illegal NotI site found at 19
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 12
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 12
    Illegal PstI site found at 26
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 41


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Categories
Parameters
None